1) Sephadex G-50 excludes molecules of molecular weight greater than 30 000 What is the predicted result if a protein mixture consisting of Albumin (Mr = 68 000) and Lysozyme (Mr = 14 000) is run through a Sephadex G-50 column?
A Lysozyme will elutefrom the column first because it is smaller than the exclusion size
B Only Albumin will elute;Lysozyme will not elute from the column at all, because it is trapped inside the beads
C Only Lysozyme willelute; Albumin will not elute from the column at all, becauseit is trapped inside the beads
D Both Albumin andLysozyme will elute from the column at the same time
E Albumin will elute from the column first because it is larger thanthe exclusion size
2)Sephadex G-15 exclude molecules of molecular weight greater than 1 500 What is the predicted result if a protein mixture consisting of Haemoglobin (Mr = 64 000) and Ribonuclease (Mr = 12 000) is run through a Sephadex G-15 column?
A Ribonucleasewill elute from the column first because it is smaller
B Haemoglobin will elute from the column first because it is larger
C Both Haemoglobin and Ribonuclease will elute from the column at the same time because they are both larger than the exclusion size
D Both Haemoglobin andRibonuclease will elute from the column at the same time because they are both smaller than the exclusion size
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